goat polyclonal anti hif1α  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Morphological Features and HIF1-Dependent Processes in the Brain of Progeny of Female Rats Exposed to Maternal Hypoxia

doi: 10.3390/ijms27083421

Figure Lengend Snippet: Western blot analysis of HIF1α and GAPDH protein content in the fetal placenta (FP) and embryonic brain of Control and PMH groups at embryonic days e14, e16, e18, and e20. ( A ) Quantification of HIF1α protein content in the fetal placenta. Data were analyzed using an ordinary two-way ANOVA with Group (Control vs. PMH) and Time (e14, e16, e18, e20) as factors. The analysis revealed a significant Group × Time interaction (F(3,32) = 4.638, p = 0.0084) and a significant main effect of Time (F(3,32) = 4.638, p = 0.0084), while the main effect of Group was not significant. Sidak’s multiple comparisons test (Control vs. PMH within each embryonic day) showed significantly higher placental HIF1α protein content in the PMH group at e18 (adjusted p = 0.0014), with no significant differences at e14, e16, or e20. ** adjusted p < 0.01. Representative immunoblots are shown below the graph. ( B ) Quantification of GAPDH protein content in the fetal placenta. No significant differences between the Control and PMH groups were detected at any examined developmental stage. ( C ) Quantification of HIF1α protein content in the embryonic brain. No significant differences between the Control and PMH groups were detected at any examined developmental stage. Representative immunoblots are shown below the graph. ( D ) Quantification of GAPDH protein content in the embryonic brain. No significant differences between the Control and PMH groups were detected at any examined developmental stage. Representative immunoblots are shown below the graph. Bars represent mean ± SEM; dots indicate individual values, n = 5 per group.

Article Snippet: Membranes were blocked for 1 h in PBS containing 5% skimmed milk and incubated for 2 h at room temperature with rabbit primary antibodies against HIF1α (1:2000, AF1009, Affinity Biosciences, Cincinnati, OH, USA), LDHA (1:5000, AF7672, Affinity Biosciences, USA), G6PD (1:2000, DF6444, Affinity Biosciences, USA), GAPDH (1:5000, AF7021, Affinity Biosciences, USA), or β-Tubulin (1:5000, AF7011, Affinity Biosciences, USA).

Techniques: Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Morphological Features and HIF1-Dependent Processes in the Brain of Progeny of Female Rats Exposed to Maternal Hypoxia

doi: 10.3390/ijms27083421

Figure Lengend Snippet: qRT-PCR analysis of Hif1α and HIF1-dependent mRNA expression in adult brain structures of Control and PMH groups. ( A ) Relative Hif1α mRNA expression in the HPC, PFC, AMG, NAcc, VTA, and RN. PMH rats showed increased Hif1α mRNA expression in the HPC (Mann–Whitney U test, p = 0.0317) and decreased Hif1α mRNA expression in the VTA (unpaired Student’s t -test, p = 0.0336), while no significant differences were detected in the other analyzed structures. * adjusted p < 0.05 ( B ) Relative Gapdh mRNA expression in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( C ) Relative Hk1 mRNA expression in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( D ) Relative Ldha mRNA expression in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( E ) Relative G6pd mRNA expression in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. Bars represent mean ± SEM; dots indicate individual values, n = 5 per group.

Article Snippet: Membranes were blocked for 1 h in PBS containing 5% skimmed milk and incubated for 2 h at room temperature with rabbit primary antibodies against HIF1α (1:2000, AF1009, Affinity Biosciences, Cincinnati, OH, USA), LDHA (1:5000, AF7672, Affinity Biosciences, USA), G6PD (1:2000, DF6444, Affinity Biosciences, USA), GAPDH (1:5000, AF7021, Affinity Biosciences, USA), or β-Tubulin (1:5000, AF7011, Affinity Biosciences, USA).

Techniques: Quantitative RT-PCR, Expressing, Control, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Morphological Features and HIF1-Dependent Processes in the Brain of Progeny of Female Rats Exposed to Maternal Hypoxia

doi: 10.3390/ijms27083421

Figure Lengend Snippet: Western blot analysis of HIF1-related protein content in adult brain structures of Control and PMH groups. ( A ) Quantification of HIF1α protein content in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( B ) Quantification of GAPDH protein content in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( C ) Quantification of LDHA protein content in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( D ) Quantification of G6PD protein content in the HPC, PFC, AMG, NAcc, VTA, and RN. No significant differences between the Control and PMH groups were detected in any analyzed brain region. ( E ) Representative immunoblots for the analyzed proteins in the corresponding brain structures. Bars represent mean ± SEM; dots indicate individual values, n = 5 per group.

Article Snippet: Membranes were blocked for 1 h in PBS containing 5% skimmed milk and incubated for 2 h at room temperature with rabbit primary antibodies against HIF1α (1:2000, AF1009, Affinity Biosciences, Cincinnati, OH, USA), LDHA (1:5000, AF7672, Affinity Biosciences, USA), G6PD (1:2000, DF6444, Affinity Biosciences, USA), GAPDH (1:5000, AF7021, Affinity Biosciences, USA), or β-Tubulin (1:5000, AF7011, Affinity Biosciences, USA).

Techniques: Western Blot, Control